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커피관장 해독법에 관한 탐구 2020.02.03해당카페글 미리보기
of the supernatant (600 μL) was removed and evaporated to be vacuum dried for 2 h at 60°C. The residue was reconstituted with 50 μL of mobile phase B and then vortex spun for 20 seconds. Five μL of this solution was injected into the...
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Re: 아연의 항염증 효과 논문 2020.02.01해당카페글 미리보기
and methods GC frass Fecal remnants (frass) were collected from German cockroaches (Blattella germanica) and reconstituted as previously described in endotoxin-free PBS [11]. The frass preparation was frozen in aliquots and the same...
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Re:The Role of Mitochondria in Apoptosis 2020.01.29해당카페글 미리보기
free system using extracts from healthy nonapoptotic cells (86). Second, in vitro apoptosome activity has been reconstituted with recombinant Cyt c, Apaf-1, and caspase-9 proteins in the presence of ATP/dATP (84). Cyt c deficient mice...
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Re:케톤체의 항암효과 β-hydroxybutyrate paradox 2020.01.29해당카페글 미리보기
of 6% perchloric acid and centrifuged at 1000 g for 10 mins. The supernatants were neutralised, freeze-dried, reconstituted in deuterium oxide and transferred to 5-mm NMR tubes. MR spectra were acquired at room temperature on a 600-MHz...
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Re:Mitochondrial uncoupling in cancer cells: Liabilities and opportunities 2020.01.18해당카페글 미리보기
that UCP2 promotes the cataplerotic efflux of these C4 metabolites out of the mitochondria. Moreover, UCP2 reconstituted in liposomes and expressed in yeast revealed that TCA intermediates are exchanged for cytosolic phosphate by a...
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Re:Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia 2019 네이처 논문 2020.01.18해당카페글 미리보기
and then supplemented with labeled-glucose or labeled-glutamine, as indicated in the experiments. All the reconstituted experimental media finally contained 10 mM glucose, 1 mM glutamine, and/or 10 mM aspartate if used. Cells were grown...
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Re: 2019 노벨생리의학상, 세포의 산소 적응 연구 2020.01.09해당카페글 미리보기
K., Czyzyk-Krzeska, M., Conaway, R.C., and Conaway, J.W. (2000). Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex. Proc Natl Acad Sci U S A 97, 10430-10435. Kibel, A., Iliopoulos...
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Re:Methylation-dependent regulation of HIF-1α stability restricts retinal and tumour angiogenesis - 백성희 교수 논문 2019.12.25해당카페글 미리보기
and Lsd1−/− MEFs were compared in the presence or absence of hypoxic challenge for 6 h. (f) Lsd1−/− MEFs were reconstituted with either WT or a catalytically inactive MT (K661A) of LSD1. HIF-1α protein levels of nuclear fraction...
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염증치료에서 꼭 알아야 할 히스타민 증후군!! 2019.12.22해당카페글 미리보기
decreased in mast cell–deficient mice and their circadian variations were lost in mast cell–deficient mice reconstituted with bone marrow–derived mast cells (BMMCs) harboring a mutation in the circadian gene Clock. Clock temporally...
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Re:Re: Mitochondrial Dysfunction in Parkinson's Disease - 논문 읽어야 2019.12.19해당카페글 미리보기
1 cleavage [77]. Fibroblasts derived from PARL deficient mice show an altered PINK1 cleavage pattern that can be reconstituted by reintroducing wild-type PARL, but not by a catalytically inactive PARL mutant [78]. However, PARL seems not...