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was obtained. Suspensions were then allowed to stand overnight (14 h). Resulting samples were subjected to centrifugation and sediments were collected and dried in a vacuum oven (−0.095 MPa, D2F-6020AF, Tianjin GongXing Laboratory...

Tewksbury, MA). Strainers were gently rinsed with 5 mL 1 × PBS to obtain a single-cell suspension, followed by centrifugation at 300g for 10 minutes at 4°C, and the supernatant were discarded. Cells were washed 2 times with 5 mL 1 ×...

at 14,000 × g for 30 min. The pellets were suspended using extraction buffer B and incubated for 20 min. After centrifugation at 14,000 × g for 5 min, the supernatant was used as membranous fraction. Western blot analysis Equal amounts...

differential centrifugation을 배우고 궁금한 점이 있어서 질문드립니다. 동물 세포의 경우 1000g->(핵)->20000g->(mt)->80000g->(소포체/골지체)->15만g->리보솜 이렇게 분리 되잖아요 그런데 이걸보다가 앞에서 배운 pulse chase 기법이 생각났습니다...

strand is termed the light strand (or L strand). The weight difference allows the two strands to be separated by centrifugation. mtDNA has one long non-coding stretch known as the non-coding region (NCR), which contains the heavy strand...

hours post mortem (Azevedo et al., 2009; Andrade-Moraes et al., 2013), detergent-assisted mechanical dissociation, centrifugation to collect nuclei in the pellet, visualization of nuclei with a fluorescent nuclear stain (4′,6-Diamidino...

dead and detached cells. Cells were then trypsinized and collected in complete medium. They were collected by centrifugation at 1500 rpm for 2–5 min and washed twice with PBS. Cell viability was assessed by trypan blue staining and cell...

arrows) stained among leukocytes. (C, D) Primo-microcells (P-MCs), 1−2 μm in size, separated by differential centrifugation, DNA-containing P-MCs selected; two microcells, one on right stained with Acridine orange. Scale bars, 10 μ...

when their number is very scarce or their function severely impaired. Blood components are usually prepared by centrifugation. Centrifuge force makes the red cells, leukocytes, plasma and platelets form different layers into the blood...