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Re: Re: Re: 글루타치온이 막망 상피세포를 치료하는 효과 2022.09.15해당카페글 미리보기
method was used to screen for differentially expressed genes 80. Western blotting Cells were plated, grown to 70% confluency, and then subjected to indicated treatments. Cells were lysed at 4 °C in a radioimmunoprecipitation assay (RIPA...
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Re:Re:Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation - 2016 네이처 논문 2020.03.25해당카페글 미리보기
41 In brief, to induce OGD/R injury, cultured primary human astrocytes were cultured in 12-well plate. After 90% confluency, rinsed and incubated in a glucose-free Hank's balanced salt solution (HBSS) composed of 140 mM NaCl, 1.2 mM MgSO...
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Re:Re:케톤체의 암 악액질 감소기전 2020.02.03해당카페글 미리보기
mouse embryo fibroblasts were cultured in DMEM with 10% FBS. For differentiation of 3T3L1 preadipocytes, after 2 days at confluency, cells were treated with differentiation medium: DMEM containing 10% FBS, 1 μM dexamethasone, 0.5 mM...
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Re:Iron deficiency impairs contractility of human cardiomyocytes through decreased mitochondrial function 2019.12.31해당카페글 미리보기
Chemicals, Houston, TX, USA); Essential 8 medium (without Y26732) was refreshed daily. Once cultures reached 80% confluency, cells were washed with phosphate buffered saline (PBS) and differentiation was initiated (day 0) by culturing...
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22.08.24 일과보고 2022.08.24해당카페글 미리보기
am 2) 업무진행사항: 1. HD iPSCs maintenance (6-colony lines, bulk harvest cells) (1) 6-colony lies : 대부분 cell confluency 70% 이상 차지하는 것을 확인했으며 내일 24-well transfer 진행 예정입니다. (2) Bulk harvest cells : cell confluency...
