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and stored at −80°C until further analysis. Nuclei Isolation Tissue samples (1 g) were defrosted and Dounce homogenized in 10 ml lysis buffer (0.32 M sucrose, 5 mM CaCl2, 3 mM magnesium acetate, 0.1 mM EDTA, 10 mM Tris-HCl [pH 8.0...

and the Prominence ultra-fast liquid chromatography system (Shimadzu, Kyoto, Japan)61,62. The liver tissue was homogenized with 10-fold volume of phosphate-buffered saline (PBS), and centrifuged at 3,500 × g for 10 min at 4 °C to...

shut down, maybe they will increase access to extraordinary hammers, but I believe it is far from enough. The homogenized and boring mode may be improved in the future, but currently it is not enough to attract more players. At the same...

and measurement of total DNA content to calculate cell numbers; and (3) “direct enumeration” of cells in homogenized brain tissue by counting cell nuclei in suspension (a rudimentary precursor of the isotropic fractionator), and the...