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and adipose tissue was collected from the inguinal region. The adipose tissues were washed thrice with phosphate buffered saline (PBS) to remove debris and red blood cells and cut into small pieces. The obtained tissue explants were...

for classical paraffin embedding. For floating immunohistochemistry, the SF samples were washed in Phosphate Buffered Saline (PBS) and transferred in water with increasing series of cryo-preservative solution (sucrose 10%–20%–30% w/v...

nicotinamide adenine dinucleotide phosphate oxidase 4; PAI-1—plasminogen activator inhibitor-1; PBS—phosphate buffered saline; PC12—pheochromocytoma cells; PCNA—proliferating cell nuclear antigen; PDGF—platelet-derived growth factor...

일반 포스페이트 버퍼와는 다른점이 있다면 그 일반 포스페이트 버퍼에 NaCl을 첨가한게 다른점이죠 원하는 PBS가 만약 0.1M이면 0.1M KH2PO4 과 0.1M K2HPO4 를 원하는 pH에 따른 비율로 섞어주고 NaCl을 0.85% 가 되도록 넣어주는거예요 pH는 monobasic...

fixation, cells were treated with 1% hydrogen peroxide. After 20 min of incubation in blocking solution (phosphate-buffered saline (PBS) containing 1% bovine serum albumin, 0.4% Triton X-100 and 4% serum), cells were incubated with anti...

TBS-Tween 3 times for 15 min and blocked after transferring 2 h at room temperature in 10% skim milk (in phosphate buffered saline, pH 7.2, containing 0.1% Tween 20). Quantitative real-time polymerase chain reaction (RT-qPCR) Total RNA...

chamber. DAPI, 4,6-diamidino-2-phenylindole; Fr, fraction; NeuN+, neuronal nuclear antigen positive; PBS, phosphate-buffered saline; Vol, volume; Modified from Herculano-Houzel and Lent (2005), Bahney and von Bartheld (2014), and...

The procedure to obtain concentrated AcSBV was conducted as described by Bailey (9) but with dilution with phosphate-buffered saline (PBS) (pH 7.4 ± 0.2). SBV 분리종은 579-bp RdRp 유전자 서열의 정렬을 기반으로, 동양종 꿀벌, 즉 AcSBV를...

자동핵산 추출기 MagNA pure LC 1.0 (Roche Diagnostics Corp,. Japan)를 이용하여 충분히 성장한 진균의 집락을 phosphate buffered saline (PBS) 완충액에 충분한 량을 풀어서 MagNA pure LC total nucleic acid isolation kit에서 제공하는 bacterial...