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20 μL of serum was mixed with an acid buffer solution (pH 4.8) in a cuvette. Subsequently, a chromogen for d-ROMS (reagent containing, N,N-diethyl-p-phenylenediamine, 20-μL) was added and mixed, resulting in oxidation of the chromogen...

acid, acetic acid, sodium chloride (troche) and sodium hydroxide (troche) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Modified eagle’s medium was obtained from (MEM, Gibco, USA) and fetal bovine serum (FBS...

secondary antibody–labeled IgG antibody diluted in 5% nonfat milk-TBST. Enhanced chemiluminescence (ECL) detection reagent (Amersham Pharmacia Biotech, Amersham, United Kingdom) was added to the membranes for 3 minutes, and the images...

Technologies, Inc. (Coralville, IA, USA). The siHO-1 or siCon (both 40 pM) was mixed with 1 μL/well of Oligofectamine reagent (Invitrogen, Carlsbad, CA, USA) in Opti-MEM (Invitrogen) according to the manufacturer’s instructions. The...

methods. It changes the form of dissolved metal ions into solid particles to facilitate their sedimentation. The reagent coagulation (coagulant) precipitates metal ions by changing pH, electro-oxidizing potential, or co-precipitation76...

주가 : 100,000원 -> 130,000원[상향] ▷아울러 동사는 언론을 통해 자체 개발한 '파이브 프라임 캡핑 시약'(5'-capping reagent) '스마트캡'(SmartCap) 관련, 벨기에 RNA(리보핵산) 기술 플랫폼 개발 기업 '퀀툼 바이오사이언스'(이하 퀀툼)와 공급계약을...

sc-2357 antibodies, both 1:10,000). Blots were developed with enhanced chemiluminescence (Western Blotting Luminol Reagent, sc-2048). Proteins were quantified by densitometry on digitized images from the mid dynamic range using Quantity...

siRNA 트랜스펙션(transfection) HT1080 세포주의 transfection은 제조업체의 지침에 따라 DharmaFECT transfection reagent set 4(T-2004-02)를 사용하여 수행되었습니다. 간단히 말해서, 세포는 2 × 10의 밀도로 6-웰 플레이트에 파종되었습니다5 세포...

LPS)-induced RAW 264.7 mouse macrophages was evaluated by the tetrazolium-based colorimetric assay (MTT), Griess reagent assay, multiplex cytokine assay, real time reverse transcription polymerase chain reaction (RT-PCR), and Fluo...