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with ECAPKHINDR (5′-GCGAAGCTTTCAGGATCTGATATATCG-3′). The PCR products were cloned into pGEM-T (Promega, http://www.promega.com/) and fully sequenced. The fragments were then cut with BamHI and HindIII or Asp718 and HindIII...

http://www.promega.com/guides/pcr_guide/default.htm

trypsin (Promega, USA)로 37℃에서 16시간동안 처리하였다. 그 peptides 는 elution buffer [50% acetonitrile, 5% trifluoroacetic acid (TFA)]로 용해시키고, 얻어진 peptide extract는 수거하여 진공 원심분리기로 동결건조한 후 -20℃에 저장하였다...

System (Promega). All the experiments were performed in triplicate and data are representative of 3–5 experiments. 2.6. Propidium Iodide Staining To evaluate cell death, cells were analysed by flow cytometry. Cells were seeded into 10...

트립신 (Promega 사, 미국)와 하룻밤 37 ° C에서 소화. 50 % ACN (피셔에 Waltham, MA, USA)와 2.5 % TFA (시그마, 세인트 루이스, MO, USA), 펩티드의 혼합물을 5 ㎎ / ㎖이 α 아노 - (4)의 포화 용액을 사용하여 분석되었다의 추출 용매에서 추출 후...

23, Promega Corporation, Madison, WI). Locus-specific allelic spectra of these markers were determined and a consistently high level of allelic diversity was observed. A considerable number of null, duplicate and off-ladder alleles were...

http://www.jumantech.kr Microscan Systems Inc. Schwartz Electro-Optics Inc. Richard Scientific Inc. Microscope Man Schwarz Pharma Manufacturing Inc. Richard-Allan Scientific An Apogent Company Microscope Org Science Accessories Corp...

Systems (Promega) were determined in a sample of 204 unrelated healthy male individuals of Chinese Hui ethnic group living in Liaoning (northeast of China). A total of 187 haplotypes were identified, of which 174 were unique, 11 was...

Systems (Promega) were determined in a sample of 215 unrelated healthy male individuals of Chinese Tujia ethnic group living in Chongqing (Southwest of China). The gene diversity values of the Y-STRs loci ranged from 0.3757(DYS391) to...